Recombinant Human Interleukin 10 Receptor, Alpha, Fc Chimera
Cat.No. : | IL10RA-492H |
Product Overview : | Recombinant Human interleukin 10 receptor, alpha encoding the signal peptide of IL-10 receptor alpha chain was fused to the extracellular domain of human CD209L (aa 1-328), which was in turn fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells. |
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- Gene Information
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Cat. No. : | IL10RA-492H |
Description : | Interleukin 10 Receptor, Alpha, a member of the 'SIGN' family of C-type lectin receptors, is a type II membrane protein that is expressed on liver sinusoidal endothelial cells (LSEC), specialized capillary vessels that are involved in antigen presentation and hepatic immune surveillance. CD209L is also expressed by endothelial cells associated with lymph nodes and in the human lung on type II alveolar cells and endothelial cells. |
Source : | Human 293 cells. |
Amino Acid Sequence : | HGTELPSPPSKLQVSKVPSSLSQEQ SEQDAIYQNLTQLKAAVGELSEKSK LQEIYQELTQLKAAVGELPEKSKLQ EIYQELTRLKAAVGELPEKSKLQEI YQELTRLKAAVGELPEKSKLQEIYQ ELTRLKAAVGELPEKSKLQEI YQELTELKAAVGELPEKSKLQEIYQELTQ LKAAVGELPDQSKQQQIYQELTDLK TAFERLCRHCPKDWTFFQGNCYFMS NSQRNWHDSVTACQEVRAQLVVIKT AEEQNFLQLQTSRSNRFSWMGLSDL NQEGTWQWVDGSPLSPSFQRYWNSG EPNNSGNEDCAEFSGSGWNDNRCDV DNYWICKKPAACFRDGIPKVDKKVE PKSCDKTHTCPPCPAPELLGGPSVF LFPPKPKDTLMISRTPEVTCVVVDV SHEDPEVKFNWYVDGVEVHNAKTKP REEQYNSTYRVVSVLTVLHQDWLNG KEYKCRVSNKALPAPIEKTISKAKG QPREPQVYTLPPSRDELTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNY KTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSL SLSPGK. |
Molecular Mass : | Interleukin 10 Receptor, Alpha Chimera migrates as a broad band between 70 and 85 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. |
pI : | Interleukin 10 Receptor, Alpha Chimera separates into a number of isoforms in 2D PAGE due to post-translational modifications, in particular glycosylation. The pI range is between 5.4 and 6.5. |
% Carbohydrate : | Interleukin 10 Receptor, Alpha Chimera consists of 5-25% carbohydrate by weight. |
Glycosylation : | Interleukin 10 Receptor, Alpha Chimera has N-linked and may have O-linked oligosaccharides. |
Purity : | >95%, as determined by SDS-PAGE and visualized by silver stain. |
Formulation : | The product is supplied as a 200ug/ml solution in sterile phosphate-buffered saline. |
Storage : | Short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
Gene Name : | IL10RA interleukin 10 receptor, alpha [ Homo sapiens ] |
Synonyms : | IL10RA; interleukin 10 receptor, alpha; IL10R; CDW210A; HIL-10R; IL-10R1;Interleukin-10 receptor alpha chain;IL-10R-A; interleukin 10 receptor, alpha; CDw210a antigen |
Gene ID : | 3587 |
mRNA Refseq : | NM_001558 |
Protein Refseq : | NP_001549 |
UniProt ID : | Q13651 |
Chromosome Location : | 11q23 |
MIM : | 146933 |
Pathway : | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |
Function : | interleukin-10 receptor activity; protein binding; receptor activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionSurface plasmon resonance and isothermal titration calorimetry techniques quantify binding kinetics between IL10RA and IL-10, revealing affinity and association/dissociation rates.
Cutting-edge flow cytometry and single-cell transcriptomics uncover the expression patterns of IL10RA across diverse immune cell subsets.
NMR spectroscopy captures dynamic interactions, revealing the flexibility and movements of IL10RA and IL-10 during their binding events.
Surface proteomics and mass spectrometry identify proteins physically interacting with IL10RA, providing insights into its complex functional networks.
Proximity labeling and quantitative proteomics unveil dynamic changes in IL10RA-associated proteins, shedding light on response mechanisms.
X-ray crystallography and cryo-electron microscopy provide detailed structural insights into the changes in IL10RA's 3D arrangement upon binding to IL-10.
Site-directed mutagenesis and alanine scanning pinpoint key amino acids in IL10RA essential for IL-10 interaction, aiding in understanding binding determinants.
Genome-wide CRISPR screening identifies genes and pathways affecting IL10RA expression and function, aiding in understanding its regulatory network.
Co-immunoprecipitation and FRET techniques elucidate the assembly of multi-protein IL10RA-IL-10 receptor complexes in cellular environments.
CRISPR/Cas9-modified cell lines and gene knockout models help dissect intricate signaling pathways activated by IL10RA upon ligand engagement.
Customer Reviews (3)
Write a reviewGood for receptor binding studies.
Consistent in bioactivity.
Suitable for ELISA.
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