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Recombinant Human Interleukin 10 Receptor, Alpha, Fc Chimera

Cat.No. : IL10RA-492H
Product Overview : Recombinant Human interleukin 10 receptor, alpha encoding the signal peptide of IL-10 receptor alpha chain was fused to the extracellular domain of human CD209L (aa 1-328), which was in turn fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells.
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Cat. No. : IL10RA-492H
Description : Interleukin 10 Receptor, Alpha, a member of the 'SIGN' family of C-type lectin receptors, is a type II membrane protein that is expressed on liver sinusoidal endothelial cells (LSEC), specialized capillary vessels that are involved in antigen presentation and hepatic immune surveillance. CD209L is also expressed by endothelial cells associated with lymph nodes and in the human lung on type II alveolar cells and endothelial cells.
Source : Human 293 cells.
Amino Acid Sequence : HGTELPSPPSKLQVSKVPSSLSQEQ SEQDAIYQNLTQLKAAVGELSEKSK LQEIYQELTQLKAAVGELPEKSKLQ EIYQELTRLKAAVGELPEKSKLQEI YQELTRLKAAVGELPEKSKLQEIYQ ELTRLKAAVGELPEKSKLQEI YQELTELKAAVGELPEKSKLQEIYQELTQ LKAAVGELPDQSKQQQIYQELTDLK TAFERLCRHCPKDWTFFQGNCYFMS NSQRNWHDSVTACQEVRAQLVVIKT AEEQNFLQLQTSRSNRFSWMGLSDL NQEGTWQWVDGSPLSPSFQRYWNSG EPNNSGNEDCAEFSGSGWNDNRCDV DNYWICKKPAACFRDGIPKVDKKVE PKSCDKTHTCPPCPAPELLGGPSVF LFPPKPKDTLMISRTPEVTCVVVDV SHEDPEVKFNWYVDGVEVHNAKTKP REEQYNSTYRVVSVLTVLHQDWLNG KEYKCRVSNKALPAPIEKTISKAKG QPREPQVYTLPPSRDELTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNY KTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSL SLSPGK.
Molecular Mass : Interleukin 10 Receptor, Alpha Chimera migrates as a broad band between 70 and 85 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation.
pI : Interleukin 10 Receptor, Alpha Chimera separates into a number of isoforms in 2D PAGE due to post-translational modifications, in particular glycosylation. The pI range is between 5.4 and 6.5.
% Carbohydrate : Interleukin 10 Receptor, Alpha Chimera consists of 5-25% carbohydrate by weight.
Glycosylation : Interleukin 10 Receptor, Alpha Chimera has N-linked and may have O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualized by silver stain.
Formulation : The product is supplied as a 200ug/ml solution in sterile phosphate-buffered saline.
Storage : Short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Gene Name : IL10RA interleukin 10 receptor, alpha [ Homo sapiens ]
Synonyms : IL10RA; interleukin 10 receptor, alpha; IL10R; CDW210A; HIL-10R; IL-10R1;Interleukin-10 receptor alpha chain;IL-10R-A; interleukin 10 receptor, alpha; CDw210a antigen
Gene ID : 3587
mRNA Refseq : NM_001558
Protein Refseq : NP_001549
UniProt ID : Q13651
Chromosome Location : 11q23
MIM : 146933
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Function : interleukin-10 receptor activity; protein binding; receptor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How are innovative experimental techniques like surface plasmon resonance and isothermal titration calorimetry utilized to study the binding kinetics between IL-10RA and its ligand IL-10? 09/23/2022

Surface plasmon resonance and isothermal titration calorimetry techniques quantify binding kinetics between IL10RA and IL-10, revealing affinity and association/dissociation rates.

How are cutting-edge flow cytometry and single-cell transcriptomics techniques applied to dissect the heterogeneity in IL10RA expression across different immune cell subsets? 12/28/2021

Cutting-edge flow cytometry and single-cell transcriptomics uncover the expression patterns of IL10RA across diverse immune cell subsets.

How do researchers employ biophysical techniques like nuclear magnetic resonance (NMR) spectroscopy to unravel the dynamic interactions between IL10RA and IL-10 at the molecular level? 05/03/2021

NMR spectroscopy captures dynamic interactions, revealing the flexibility and movements of IL10RA and IL-10 during their binding events.

Could you provide insights into the comprehensive experimental methodologies, such as surface proteomics and mass spectrometry, used to identify potential IL10RA-interacting partners in complex cellular environments? 04/27/2021

Surface proteomics and mass spectrometry identify proteins physically interacting with IL10RA, providing insights into its complex functional networks.

What innovative approaches, such as proximity labeling and quantitative proteomics, are employed to uncover dynamic changes in IL10RA-associated protein networks upon IL-10 stimulation? 07/24/2020

Proximity labeling and quantitative proteomics unveil dynamic changes in IL10RA-associated proteins, shedding light on response mechanisms.

Could you explain the complex experimental methodologies employed to investigate the conformational changes in IL10RA upon ligand binding using techniques like X-ray crystallography and cryo-electron microscopy? 04/02/2020

X-ray crystallography and cryo-electron microscopy provide detailed structural insights into the changes in IL10RA's 3D arrangement upon binding to IL-10.

What advanced strategies, such as site-directed mutagenesis and alanine scanning, are used to pinpoint the critical amino acid residues in IL10RA that are essential for its interaction with IL-10? 03/07/2019

Site-directed mutagenesis and alanine scanning pinpoint key amino acids in IL10RA essential for IL-10 interaction, aiding in understanding binding determinants.

How do researchers leverage advanced genome-wide CRISPR screening techniques to systematically identify genes and pathways that modulate IL10RA expression and function in various physiological contexts? 07/01/2018

Genome-wide CRISPR screening identifies genes and pathways affecting IL10RA expression and function, aiding in understanding its regulatory network.

Can you elaborate on the sophisticated experimental approaches, such as co-immunoprecipitation and F枚rster resonance energy transfer (FRET), used to explore the formation of IL10RA-IL-10 receptor complexes? 07/19/2017

Co-immunoprecipitation and FRET techniques elucidate the assembly of multi-protein IL10RA-IL-10 receptor complexes in cellular environments.

What state-of-the-art experimental designs, including CRISPR/Cas9-edited cell lines and gene knockout models, are harnessed to study the downstream signaling cascades initiated by IL10RA activation? 03/15/2017

CRISPR/Cas9-modified cell lines and gene knockout models help dissect intricate signaling pathways activated by IL10RA upon ligand engagement.

Customer Reviews (3)

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Reviews
02/02/2021

    Good for receptor binding studies.

    11/19/2019

      Consistent in bioactivity.

      06/19/2017

        Suitable for ELISA.

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