Recombinant Human Interleukin 12B, Fc-Tagged

• Cat.No.: IL12B-35H
• Product Overview: Recombinant Human Interleukin 12B, Fc-Tagged expressed in E.coli is a disulfide-linked homodimer. The reduced monomer consists of 544 amino acids and has a predicted molecular mass of 61.4 kDa. As a result of glycosylation, the rhIL12B/Fc monomer migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.

Specification

• Cat. No.: IL12B-35H
• Description: Interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. A DNA sequence encoding the p40 subunit of human IL12, termed asIL12B (NP_002178.2) (Met 1- Ser 328 ) was fused with the Fc region ofhuman IgG1 at the C-terminus.
• Predicted N terminal: Ile 23
• Purity: > 95%, as determined by SDS-PAGE
• Endotoxin: ﹤1.0 EU per 1μg cytokine as determined by the LAL method.
• Formulation: Supplied as a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50 mM Tris, pH 7.5
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Unitprot ID: P29460

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Synonyms: OTTHUMP00000160820; CLMF; CLMF2; IL-12B; NKSF; NKSF2; CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); IL12B
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• Chromosome Location: 5q31.1-q33.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
• Function: contributes_to cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; signal transducer activity

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.