Recombinant Human Interleukin 4 Receptor

• Cat.No.: IL4R-586H
• Product Overview: This recombinant human sIL-4R alpha is a 23.7 kDa protein containing 207 amino acid residues consisting of only the extracellular domain of the IL-4R alpha. It is produced inHEK 293 cells.

Specification

• Cat. No.: IL4R-586H
• Description: Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, yc and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids.
• Source: HEK 293 cells.
• Amino Acid Sequence: MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV FLLSEAHTCI PENNGGAGCV CHLLMDDVVS ADNYTLDLWA GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR IAASTLKSGI SYRARVRAWA QCYNTTWSEW SPSTKWHNSY REPFEQH.
• Note: If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
• Supplied: The sterile filtered solution was lyophilized from 1 x PBS, pH 7.2.
• Purity: Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
• Reconstitution: We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Allow to set at least 10 minutes. Rotate and/or vortex to mix. Centrifuge for 1-2 minutes at 1000 rpm to concentrate solution in bottom of vial.
• Reactivity: The ED₅₀as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is < 5.0 ng/ml (in the presence of 0.5 ng/ml IL-4), corresponding to a specific activity of > 2 x105 units/mg.
• Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20℃. Reconstituted IL-4R should be stored in working aliquots at -20℃. Avoid frequent freeze thaw cycles.
• Tag: Non

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Synonyms: IL4R; interleukin 4 receptor; CD124; IL4RA; interleukin 4 receptor alpha chain; OTTHUMP00000122506; interleukin-4 receptor alpha chain;CD_antigen;IL-4R-alpha;Soluble interleukin-4 receptor alpha chain; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394
• Chromosome Location: 16p12.1-p11.2
• Pathway: Cytokine-cytokine receptor interaction; Endocytosis; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: interleukin-4 receptor activity; receptor activity; protein binding; receptor signaling protein activity

Reviews

06/30/2022

Easy to attach to the surface of cells or materials.

09/29/2021

It has growth factor activity to promote cell growth and proliferation.

02/27/2019

In animals or humans caused by the prone production of antibodies.

Q&As

How about function of IL4R?

IL4R is a synthetic protein that mimics the IL-4 signaling pathway in nature and binds to the IL-4 receptor in cells, which can be used to study the IL-4 signaling pathway and related diseases.

What is the production technology of IL4R?

Common IL4R production technologies include prokaryotic expression systems and mammalian cell expression systems.

Which application of IL4R in tumor therapy?

This protein is used in tumor immunotherapy, which can promote anti-tumor immune response and enhance the anti-tumor effect of cytotoxic T lymphocytes.

In what fields does IL4R have applications?

It is mainly used in immunology, drug research and other fields. It is also widely used in vaccines, biotechnology, cancer treatment and so on.

Are there have application of IL4R in the treatment of asthma?

IL4R is used to treat severe pheochromocytome-induced aerodoritis and was approved by the FDA as the first drug to treat this disease.

Can IL4R be administered orally?

IL4R cannot generally be administered orally, and needs to be administered by injection or intravenous drip.