Recombinant Human Interleukin 4 Receptor

• Cat.No.: IL4R-586H
• Product Overview: This recombinant human sIL-4R alpha is a 23.7 kDa protein containing 207 amino acid residues consisting of only the extracellular domain of the IL-4R alpha. It is produced inHEK 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, yc and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids.
• Amino Acid Sequence: MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV FLLSEAHTCI PENNGGAGCV CHLLMDDVVS ADNYTLDLWA GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR IAASTLKSGI SYRARVRAWA QCYNTTWSEW SPSTKWHNSY REPFEQH.
• Note: If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
• Supplied: The sterile filtered solution was lyophilized from 1 x PBS, pH 7.2.
• Purity: Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
• Reconstitution: We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Allow to set at least 10 minutes. Rotate and/or vortex to mix. Centrifuge for 1-2 minutes at 1000 rpm to concentrate solution in bottom of vial.
• Reactivity: The ED₅₀as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is < 5.0 ng/ml (in the presence of 0.5 ng/ml IL-4), corresponding to a specific activity of > 2 x105 units/mg.
• Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20℃. Reconstituted IL-4R should be stored in working aliquots at -20℃. Avoid frequent freeze thaw cycles.

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Synonyms: IL4R; interleukin 4 receptor; CD124; IL4RA; interleukin 4 receptor alpha chain; OTTHUMP00000122506; interleukin-4 receptor alpha chain;CD_antigen;IL-4R-alpha;Soluble interleukin-4 receptor alpha chain; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394
• Chromosome Location: 16p12.1-p11.2
• Pathway: Cytokine-cytokine receptor interaction; Endocytosis; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: interleukin-4 receptor activity; receptor activity; protein binding; receptor signaling protein activity