Recombinant Human IRS2 protein, His & T7-tagged

• Cat.No.: IRS2-1051H
• Product Overview: Recombinant Human IRS2 aa. (Ser985~Ala1255 (Accession # Q9Y4H2) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser985~Ala1255
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: IRS2 insulin receptor substrate 2 [ Homo sapiens (human) ]
• Official Symbol: IRS2
• Synonyms: IRS2; insulin receptor substrate 2; IRS-2; insulin receptor substrate 2
• Gene ID: 8660
• mRNA Refseq: NM_003749.2
• Protein Refseq: NP_003740.2
• UniProt ID: Q9Y4H2