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Recombinant Human JAK2 (V617F) (JH1, JH2 Domain) Protein, GST/Avi-tagged

Cat.No. : JAK2-31H
Product Overview : Recombinant human JAK2 (Janus Kinase 2) encompassing amino acids 513-1132 (end) and containing the catalytic domain (JH1) and the pseudo kinase domain (JH2). This protein contains mutation of interest V617F. The construct also contains an N-terminal GST-tag and a C-terminal Avi-Tag™. The protein was affinity purified.
  • Specification
  • Gene Information
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Description : This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Source : HEK293
Species : Human
Tag : GST/Avi
Form : Aqueous buffer solution
Molecular Mass : 99 kDa
Protein length : 513-1132
Purity : 28%
Applications : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Usage : Assay was performed according to the TYR Phosphorylation Assay Kit on a Neutravidin Coated plate with phosphorylated JAK2 titrated from 500 ng/rxn to 0 ng/rxn.
Storage : At least 6 months at –80 centigrade.
Storage Buffer : 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20 mM GSH and 20% glycerol.
Gene Name : JAK2 Janus kinase 2 [ Homo sapiens (human) ]
Official Symbol : JAK2
Synonyms : JAK2; Janus kinase 2; JTK10; tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2 (a protein tyrosine kinase); EC 2.7.10.2; jak; jh1
Gene ID : 3717
mRNA Refseq : NM_004972
Protein Refseq : NP_004963
MIM : 147796
UniProt ID : O60674

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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