Recombinant Human KDSR protein, His & GST-tagged

• Cat.No.: KDSR-1662H
• Product Overview: Recombinant Human KDSR aa. (Lys26~Asp270 (Accession # Q06136)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene catalyzes the reduction of 3-ketodihydrosphingosine to dihydrosphingosine. The putative active site residues of the encoded protein are found on the cytosolic side of the endoplasmic reticulum membrane. A chromosomal rearrangement involving this gene is a cause of follicular lymphoma, also known as type II chronic lymphatic leukemia. The mutation of a conserved residue in the bovine ortholog causes spinal muscular atrophy.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 56.7kDa
• Protein length: Lys26~Asp270 (Accession # Q06136)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 6.2

Gene Information

• Gene Name: KDSR 3-ketodihydrosphingosine reductase [ Homo sapiens (human) ]
• Official Symbol: KDSR
• Synonyms: KDSR; 3-ketodihydrosphingosine reductase; DHSR; FVT1; EKVP4; SDR35C1; 3-ketodihydrosphingosine reductase; 3-dehydrosphinganine reductase; FVT-1; KDS reductase; follicular lymphoma variant translocation 1; follicular variant translocation protein 1; short chain dehydrogenase/reductase family 35C member 1
• Gene ID: 2531
• mRNA Refseq: NM_002035.2
• Protein Refseq: NP_002026.1
• UniProt ID: Q06136