Recombinant Human Ketohexokinase (fructokinase)

• Cat.No.: KHK-905H
• Product Overview: Ketohexokinase Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids and having a molecular mass of 32.7 kDa.

Specification

• Cat. No.: KHK-905H
• Description: Ketohexokinase catalyzes the phosphorylation of fructose to produce fructose-1-phosphate, resulting in the utilization of ATP and creation of AMP. Ketohexokinase commences initial step in the metabolism of dietary fructose and is a significant regulator of hepatic glucose metabolism. Ketohexokinase is found in liver, renal cortex, and small intestine.
• Source: Human
• Host: Escherichia Coli.
• Form: The protein solution contains 1xPBS, pH 7.4 and 10 % glycerol.
• Purity: Greater than 90.0 % as determined by SDS-PAGE.
• Amino acid sequence: MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Avoid multiple freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: KHK ketohexokinase (fructokinase) [ Homo sapiens ]
• Official Symbol: KHK
• Synonyms: KHK; ketohexokinase (fructokinase); ketohexokinase; OTTHUMP00000122595; OTTHUMP00000122596; OTTHUMP00000200951; hepatic fructokinase
• Gene ID: 3795
• mRNA Refseq: NM_000221
• Protein Refseq: NP_000212
• MIM: 614058
• UniProt ID: P50053
• Chromosome Location: 2p23.3-p23.2
• Pathway: Fructose and mannose metabolism; Fructose catabolism; Metabolic pathways; Metabolism of carbohydrates
• Function: ATP binding; ketohexokinase activity; kinase activity; nucleotide binding; protein binding; transferase activity

Reviews

12/03/2020

Due to the short half-life and high clearance characteristics of KHK, they can better adapt to the treatment needs of patients of different ages, providing patients with a safer and more effective treatment option.

12/30/2019

The stability of KHK is so good that it can be manipulated experimentally for long periods of time without compromising its performance and results.

09/19/2019

Reproducibility of the Western blot was significantly improved by using this protein.

Q&As

How is the activity of KHK protein regulated?

Activity of KHK protein is affected by many factors, including gene expression, hormone levels, nutritional status, etc. In addition, some small molecule compounds can also regulate the activity of KHK proteins.

What is the relationship between KHK protein and the occurrence and progression of disease?

Some studies have shown that abnormal expression of the KHK protein may be related to the occurrence and progression of some diseases. For example, in some cancers, the expression level of the KHK protein may change. In addition, the KHK protein may also be a drug target for the treatment of these diseases.

How to use the activity of KHK protein for disease treatment?

The activity of the KHK protein can be modulated by drugs or other small molecule compounds to treat diseases related to the KHK protein. For example, some drugs can inhibit the activity of the KHK protein to treat diseases associated with abnormal metabolism of ketone bodies

What diseases are mutations or abnormal expression of the KHK protein associated with?

Some studies have shown that mutations or abnormal expression of the KHK protein may be related to some diseases, such as cancer, neurodegenerative diseases, etc. However, the specific association needs to be confirmed by further research.

How about the interaction between the KHK protein and other enzymes or proteins?

The interaction between the KHK protein and other enzymes or proteins is complex. For example, it may interact with fatty acid synthase to co-catalyze the synthesis of ketone bodies. In addition, it may interact with some regulators to regulate its activity.

How to use KHK protein for disease diagnosis?

The occurrence, progression, and prognosis of the disease can be assessed by examining the expression level, activity, and interaction of KHK protein with other proteins. These features can also be used to develop new biomarkers and drug targets.