Active Recombinant Human KLKB1 protein, His-tagged, Low Endotoxin

• Cat.No.: KLKB1-485H
• Product Overview: Recombinant Human KLKB1 protein(P03952)(Gly20-Ala638), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-638 aa
• Form: Lyophilized from 0.22μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is > 1000 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLKB1 kallikrein B, plasma (Fletcher factor) 1 [ Homo sapiens ]
• Official Symbol: KLKB1
• Synonyms: KLKB1; kallikrein B, plasma (Fletcher factor) 1; KLK3; plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain; PPK
• Gene ID: 3818
• mRNA Refseq: NM_000892
• Protein Refseq: NP_000883
• MIM: 229000
• UniProt ID: P03952