Description : |
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His & S |
Form : |
Freeze-dried powder |
Molecular Mass : |
44kDa as determined by SDS-PAGE reducing conditions. |
Protein length : |
Glu91~Lys394 (Accession # P08729) |
Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
Purity : |
>95% |
Characteristic : |
The isoelectric point is 5. |
Applications : |
SDS-PAGE; WB; ELISA; IP. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : |
Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |