Active Recombinant Human LDLR Protein, His-Avi-tagged, Biotinylated

• Cat.No.: LDLR-267H
• Product Overview: Biotinylated Human LDL R, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ala 22 - Arg 788 (Accession # P01130-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Predicted N Terminal: Ala 22
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human PCSK9, His Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human LDL R, His Tag, Avi Tag with a linear range of 0.02-0.625 ug/mL.
• Molecular Mass: The protein has a calculated MW of 88.5 kDa. The protein migrates as 90-150 KDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LDLR low density lipoprotein receptor [ Homo sapiens ]
• Official Symbol: LDLR
• Synonyms: LDLR; low density lipoprotein receptor; low-density lipoprotein receptor; familial hypercholesterolemia; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3; FH; FHC; LDLCQ2
• Gene ID: 3949
• mRNA Refseq: NM_000527
• Protein Refseq: NP_000518
• MIM: 606945
• UniProt ID: P01130