Recombinant Human LDLR Protein, His-Avi-tagged, Biotinylated LDLR-267H

Recombinant Human LDLR Protein, His-Avi-tagged, Biotinylated

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Cat.No. : LDLR-267H Optional Service: Optional requirements on this protein
Product Overview : Biotinylated Human LDL R, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ala 22 - Arg 788 (Accession # P01130-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Ala 22
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Human PCSK9, His Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human LDL R, His Tag, Avi Tag with a linear range of 0.02-0.625 ug/mL.
Molecular Mass : The protein has a calculated MW of 88.5 kDa. The protein migrates as 90-150 KDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >80% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : LDLR low density lipoprotein receptor [ Homo sapiens ]
Official Symbol : LDLR
Synonyms : LDLR; low density lipoprotein receptor; low-density lipoprotein receptor; familial hypercholesterolemia; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3; FH; FHC; LDLCQ2;
Gene ID : 3949
mRNA Refseq : NM_000527
Protein Refseq : NP_000518
MIM : 606945
UniProt ID : P01130
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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