Recombinant Human LGALS13 293 Cell Lysate

Cat.No. : LGALS13-4768HCL
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Description : Antigen standard for lectin, galactoside-binding, soluble, 13 (LGALS13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : LGALS13 lectin, galactoside-binding, soluble, 13 [ Homo sapiens ]
Official Symbol : LGALS13
Synonyms : LGALS13; lectin, galactoside-binding, soluble, 13; galactoside-binding soluble lectin 13; galectin 13; PLAC8; PP13; gal-13; galectin-13; placental protein 13; placental tissue protein 13; beta-galactoside-binding lectin; GAL13;
Gene ID : 29124
mRNA Refseq : NM_013268
Protein Refseq : NP_037400
MIM : 608717
UniProt ID : Q9UHV8
Chromosome Location : 19q13.1
Function : carboxylesterase activity; hydrolase activity; lysophospholipase activity; sugar binding;

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