Active Recombinant Human LGALS3 protein, N-His-tagged

• Cat.No.: LGALS3-1089H
• Product Overview: Active Recombinant Human LGALS3 protein(P17931)(Ala2-Ile250) is expressed from HEK293 with His tag at the N-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 2-250 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP (pH 7.4).
• Bio-activity: Immobilized Human Galectin 3, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: LGALS3 lectin, galactoside-binding, soluble, 3 [ Homo sapiens ]
• Official Symbol: LGALS3
• Synonyms: LGALS3; lectin, galactoside-binding, soluble, 3; LGALS2; galectin-3; galectin 3; GALIG; MAC 2; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; L31; GAL3; MAC2; CBP35; GALBP
• Gene ID: 3958
• mRNA Refseq: NM_001177388
• Protein Refseq: NP_001170859
• MIM: 153619
• UniProt ID: P17931