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Active Recombinant Human LIF protein, His-Avi-tagged, Biotinylated

Cat.No. : LIF-420H
Product Overview : Biotinylated Recombinant Human LIF protein(Ser 23 - Phe 202), fused with His and Avi tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His-Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Biotinylated Human LIF, His,Avitag on SA-coated surface at 1 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag with a linear range of 1-31 ng/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 23.4 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength : Ser23-Phe202
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : LIF leukemia inhibitory factor [ Homo sapiens ]
Official Symbol : LIF
Synonyms : LIF; leukemia inhibitory factor; CDF; cholinergic differentiation factor; DIA; differentiation inhibitory activity; differentiation inducing factor; hepatocyte stimulating factor III; HILDA; human interleukin in DA cells; D factor; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; differentiation-stimulating factor; MLPLI;
Gene ID : 3976
mRNA Refseq : NM_001257135
Protein Refseq : NP_001244064
MIM : 159540
UniProt ID : P15018

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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