Recombinant Human LIF Protein, His-Avi-tagged, Biotinylated

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Recombinant Human LIF Protein, His-Avi-tagged, Biotinylated

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Cat.No. : LIF-420H
Product Overview : Biotinylated Human LIF, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ser 23 - Phe 202 (Accession # P15018-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Ser 23
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 23.4 kDa. The protein migrates as 37-45 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : LIF leukemia inhibitory factor [ Homo sapiens ]
Official Symbol : LIF
Synonyms : LIF; leukemia inhibitory factor; CDF; cholinergic differentiation factor; DIA; differentiation inhibitory activity; differentiation inducing factor; hepatocyte stimulating factor III; HILDA; human interleukin in DA cells; D factor; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; differentiation-stimulating factor; MLPLI;
Gene ID : 3976
mRNA Refseq : NM_001257135
Protein Refseq : NP_001244064
MIM : 159540
UniProt ID : P15018

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