"LIF" Related Products

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Recombinant Human LIF Protein, His-Avi-tagged, Biotinylated

Cat.No.: LIF-420H
Product Overview: Biotinylated Human LIF, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ser 23 - Phe 202 (Accession # P15018-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source: HEK293
Species: Human
Tag : His-Avi
Predicted N Terminal: Ser 23
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass: The protein has a calculated MW of 23.4 kDa. The protein migrates as 37-45 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: LIF leukemia inhibitory factor [ Homo sapiens ]
Official Symbol: LIF
Synonyms: LIF; leukemia inhibitory factor; CDF; cholinergic differentiation factor; DIA; differentiation inhibitory activity; differentiation inducing factor; hepatocyte stimulating factor III; HILDA; human interleukin in DA cells; D factor; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; differentiation-stimulating factor; MLPLI;
Gene ID: 3976
mRNA Refseq: NM_001257135
Protein Refseq: NP_001244064
MIM: 159540
UniProt ID: P15018

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