Recombinant Human LILRB1 protein, Fc-tagged

• Cat.No.: LILRB1-110H
• Product Overview: Recombinant Human LILRB1 protein (Gly24-His458), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: CD85 antigen-like family member J (CD85J) is also known as Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1 or LIR-1), Immunoglobulin-like transcript 2 (ILT-2), Monocyte/macrophage immunoglobulin-like receptor 7 (MIR7), which belongs to leukocyte immunoglobulin-like receptor (LIR) family. CD85J/LILRB1 Contains 4 Ig-like C2-type (immunoglobulin-like) domains. CD85J/ LIR-1 is expressed predominantly on B-cells and monocytes. CD85J is receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles and is also receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. CD85J/LILRB1 interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. CD85J/LILRB1 inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calciumions.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 73.8 kDa. The protein migrates as 90-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 435
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LILRB1
• Official Symbol: LILRB1
• Synonyms: LILRB1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; leukocyte immunoglobulin-like receptor subfamily B member 1; CD85; CD85j; ILT2; LIR 1; LIR1; MIR 7; ILT-2; CD85 antigen; Ig-like transcript 2; leukocyte Ig-like receptor-1; immunoglobulin-like transcript 2; CD85 antigen-like family member J; leukocyte immunoglobulin-like receptor 1; monocyte/macrophage immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; MIR7; CD85J; LIR-1; MIR-7; FLJ37515
• Gene ID: 10859
• mRNA Refseq: NM_001081637
• Protein Refseq: NP_001075106
• MIM: 604811
• UniProt ID: Q8NHL6