Description : |
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
72.4 kDa |
AA Sequence : |
MRLTLLCCTWREERMGEEGSELPVC ASCGQRIYDGQYLQALNADWHADCF RCCDCSASLSHQYYEKDGQLFCKKD YWARYGESCHGCSEQITKGLVMVAG ELKYHPECFICLTCGTFIGDGDTYT LVEHSKLYCGHCYYQTVVTPVIEQI LPDSPGSHLPHTVTLVSIPASSHGK RGLSVSIDPPHGPPGCGTEHSHTVR VQGVDPGCMSPDVKNSIHVGDRILE INGTPIRNVPLDEIDLLIQETSRLL QLTLEHDPHDTLGHGLGPETSPLSS PAYTPSGEAGSSARQKPVLRSCSID RSPGAGSLGSPASQRKDLGRSESLR VVCRPHRIFRPSDLIHGEVLGKGCF GQAIKVTHRETGEVMVMKELIRFDE ETQRTFLKEVKVMRCLEHPNVLKFI GVLYKDKRLNFITEYIKGGTLRGII KSMDSQYPWSQRVSFAKDIASGMAY LHSMNIIHRDLNSHNCLVRENKNVV VADFGLARLMVDEKTQPEGLRSLKK PDRKKRYTVVGNPYWMAPEMINGRS YDEKVDVFSFGIVLCEIIGRVNADP DYLPRTMDFGLNVRGFLDRYCPPNC PPSFFPITVRCCDLDPEKRPSFVKL EHWLETLRMHLAGHLPLGPQLEQLD RGFWETYRRGESGLPAHPEVPDTRT RPLEQKLISEEDLAANDILDYKDDD DKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |