Recombinant Human LIPA Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: LIPA-6304H
• Product Overview: LIPA MS Standard C13 and N15-labeled recombinant protein (NP_000226) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 45.4 kDa
• AA Sequence: MKMRFLGLVVCLVLWPLHSEGSGGK LTALDPETNMNVSEIISYWGFPSEE YLVETEDGYILCLNRIPHGRKNHSD KGPKPVVFLQHGLLADSSNWVTNLA NSSLGFILADAGFDVWMGNSRGNTW SRKHKTLSVSQDEFWAFSYDEMAKY DLPASINFILNKTGQEQVYYVGHSQ GTTIGFIAFSQIPELAKRIKMFFAL GPVASVAFCTSPMAKLGRLPDHLIK DLFGDKEFLPQSAFLKWLGTHVCTH VILKELCGNLCFLLCGFNERNLNMS RVDVYTTHSPAGTSVQNMLHWSQAV KFQKFQAFDWGSSAKNYFHYNQSYP PTYNVKDMLVPTAVWSGGHDWLADV YDVNILLTQITNLVFHESIPEWEHL DFIWGLDAPWRLYNKIINLMRKYQT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: LIPA lipase A, lysosomal acid type [ Homo sapiens (human) ]
• Official Symbol: LIPA
• Synonyms: LIPA; lipase A, lysosomal acid, cholesterol esterase; lysosomal acid lipase/cholesteryl ester hydrolase; CESD; LAL; Wolman disease; sterol esterase; cholesteryl esterase; lysosomal acid lipase; cholesterol ester hydrolase; acid cholesteryl ester hydrolase
• Gene ID: 3988
• mRNA Refseq: NM_000235
• Protein Refseq: NP_000226
• MIM: 613497
• UniProt ID: P38571