Recombinant Human LRRK2 protein, His-tagged

• Cat.No.: LRRK2-1553H
• Product Overview: Recombinant Human LRRK2 aa. (Phe1479~Pro1729 (Accession # Q5S007)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.0kDa
• Protein length: Phe1479~Pro1729 (Accession # Q5S007)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 7.8

Gene Information

• Gene Name: LRRK2 leucine rich repeat kinase 2 [ Homo sapiens (human) ]
• Official Symbol: LRRK2
• Synonyms: LRRK2; leucine rich repeat kinase 2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN; leucine-rich repeat serine/threonine-protein kinase 2; augmented in rheumatoid arthritis 17
• Gene ID: 120892
• mRNA Refseq: NM_198578.3
• Protein Refseq: NP_940980.3
• UniProt ID: Q5S007