Active Recombinant Human LRRK2 Protein, FLAG-tagged

• Cat.No.: LRRK2-33H
• Product Overview: Recombinant full length Human LRRK2 protein was expressed in mammalian cells with DYKDDDDK-tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Flag
• Protein Length: 1-2527 aa
• Description: Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene, which codes for a multi-domain protein kinase, are considered the most common genetic cause of late-onset autosomal-dominant Parkinson's disease. This full-length, purified and active wild-type LRRK2 protein may be useful in research to better understand the native protein, unaffected by mutations, as well as to explore the mechanisms underlying the disease.
• Form: 50 mM Tris (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X–100, 2 mM DTT and 50% Glycerol.
• Bio-activity: 61 nmoles of phosphate transferred to ERM (LRRKtide) peptide substrate (RLGRDKYKTLRQIRQ) per minute per mg of total protein at 30 centigrade. Activity determined at a final protein concentration of 2.08 µg/mL.
• Molecular Mass: 288 kDa
• Purity: 85% as determined by a Coomassie blue stained SDS-PAGE gel
• Storage: Store at -80 centigrade
• Concentration: 0.22 mg/mL total protein as measured using the Bradford protein assay with BSA as a standard.
• Full Length: Full L.
• Publications:
  Oligomerization of Lrrk controls actin severing and α-synuclein neurotoxicity in vivo (2021)

Gene Information

• Gene Name: LRRK2 leucine-rich repeat kinase 2 [ Homo sapiens ]
• Official Symbol: LRRK2
• Synonyms: LRRK2; leucine-rich repeat kinase 2; PARK8, Parkinson disease (autosomal dominant) 8; leucine-rich repeat serine/threonine-protein kinase 2; DKFZp434H2111; FLJ45829; RIPK7; augmented in rheumatoid arthritis 17; PARK8; AURA17; DARDARIN
• Gene ID: 120892
• mRNA Refseq: NM_198578
• Protein Refseq: NP_940980
• MIM: 609007
• UniProt ID: Q5S007