Active Recombinant Human LRRK2 Protein, FLAG-tagged

• Cat.No.: LRRK2-33H
• Product Overview: Recombinant full length Human LRRK2 protein was expressed in mammalian cells with DYKDDDDK-tag.

Specification

• Description: Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene, which codes for a multi-domain protein kinase, are considered the most common genetic cause of late-onset autosomal-dominant Parkinson's disease. This full-length, purified and active wild-type LRRK2 protein may be useful in research to better understand the native protein, unaffected by mutations, as well as to explore the mechanisms underlying the disease.
• Source: Mammalian cells
• Species: Human
• Tag: FLAG
• Form: 50 mM Tris (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X–100, 2 mM DTT and 50% Glycerol.
• Bio-activity: 61 nmoles of phosphate transferred to ERM (LRRKtide) peptide substrate (RLGRDKYKTLRQIRQ) per minute per mg of total protein at 30 centigrade. Activity determined at a final protein concentration of 2.08 µg/mL.
• Molecular Mass: 288 kDa
• Purity: 85% as determined by a Coomassie blue stained SDS-PAGE gel
• Storage: Store at -80 centigrade
• Concentration: 0.22 mg/mL total protein as measured using the Bradford protein assay with BSA as a standard.

Gene Information

• Gene Name: LRRK2 leucine-rich repeat kinase 2 [ Homo sapiens ]
• Official Symbol: LRRK2
• Synonyms: LRRK2; leucine-rich repeat kinase 2; PARK8, Parkinson disease (autosomal dominant) 8; leucine-rich repeat serine/threonine-protein kinase 2; DKFZp434H2111; FLJ45829; RIPK7; augmented in rheumatoid arthritis 17; PARK8; AURA17; DARDARIN
• Gene ID: 120892
• mRNA Refseq: NM_198578
• Protein Refseq: NP_940980
• MIM: 609007
• UniProt ID: Q5S007

Reviews

08/07/2022

It has an inhibitory or killing effect on specific pathogens.

10/26/2019

High sensitivity and specificity were found in biosensor preparation.

02/14/2019

Good selectivity for specific disease-related targets.

Q&As

What is the relationship between LRRK2 gene mutations and neurodevelopment?

LRRK2 gene mutations may play an important role in neurodevelopment, including neural precursor cell fate determination, cell migration, and axon clumping.

What are the effects of LRRK2 gene mutations on other diseases?

In addition to Parkinson's disease, LRRK2 gene mutations are also associated with an increased risk of other diseases, such as Crohn's disease, leukemia and cancer.

How to assess the prognostic impact of LRRK2 gene mutations on patients with Parkinson's disease?

By analyzing the clinical and genetic data of a large number of patients, the impact of LRRK2 gene mutations on the prognosis of Parkinson's disease patients, such as age of onset, clinical symptoms, and disease progression, can be evaluated.

How do mutations in LRRK2 affect Parkinson's disease?

LRRK2 gene mutations may complicate the pathogenesis of Parkinson's disease, including increased apoptosis, affecting vesicles, mitochondria, and autophagy function.

Do drugs that inhibit LRRK2 proteins have potential value in treating Parkinson's disease?

Drugs that inhibit LRRK2 protein have potential value in the treatment of Parkinson's disease, but are still in clinical research and further research is needed on their efficacy and safety.

What are the ways to study the function and regulation of LRRK2 protein?

The function and regulation of LRRK2 can be studied using different research methods such as cell culture models, mouse models, fruit fly models, and human induced pluripotent stem cells.