Active Recombinant Human LTA protein, His/Avi-tagged, Biotinylated

• Cat.No.: LTA-051H
• Product Overview: Biotinylated Recombinant Human LTA(Leu35-Leu205) protein, fused to His/Avi tag at the C-terminus, was expressed in HEK293 cells .

Specification

• Description: Lymphotoxin alpha (LT-α), and Lymphotoxin beta (LT-β) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development
  (1, 2). The 25 kDa mature human LT-α is a secreted protein that shares 75% amino acid (aa) sequence identity with mouse and rat LT-α (3, 4). The 33 kDa mature
  human LT-β is a type II transmembrane protein that shares 73% aa sequence identity with mouse and rat LT-β within common regions of their extracellular domains
  (5). Relative to the human protein, mouse and rat LT-β have a 66 aa or 65 aa insertion within the ECD, respectively. LT-α can be secreted as a homotrimer that binds
  and activates TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, HVEM/TNFRSF14, and Troy/TNFRSF19 (6-8). LT-α is required for development of the spleen, lymph nodes,
  and Peyer’s patches (9). It also regulates T cell homing to the gut and IgA induction (10). In addition, LT-α can form membrane-associated heterotrimers with two
  copies of LT-β on the surface of B, T, LTi, and ILC3 cells (2, 5, 11). The Lymphotoxin α1/β2 heterotrimer binds and activates the Lymphotoxin beta R/TNFRSF3
  (LTβR) which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules
  (HEV) (2, 12, 13). LTβR also serves as a receptor for LIGHT/TNFSF14 (14). LT-α1/β2 promotes the development of FDC networks and HEV in lymphoid tissue, the
  class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (10, 15-17). It can be shed by ADAM17 or MMP-8 mediated
  cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (18).
• Source: HEK293 cells
• Species: Human
• Tag: His/Avi
• Predicted N Terminal: Leu35
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.
  When Recombinant Human Lymphotoxin beta R Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Lymphotoxin LTA His-tag Avi-tag that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.
• Molecular Mass: 69-80 kDa
• Protein length: Leu35-Leu205
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: LTA lymphotoxin alpha (TNF superfamily, member 1) [ Homo sapiens ]
• Official Symbol: LTA
• Synonyms: LTA; lymphotoxin alpha (TNF superfamily, member 1); TNFB; lymphotoxin-alpha; LT; TNFSF1; LT-alpha; TNF-beta; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
• Gene ID: 4049
• mRNA Refseq: NM_000595
• Protein Refseq: NP_000586
• MIM: 153440
• UniProt ID: P01374