Recombinant Human lysyl oxidase, His-tagged

• Cat.No.: LOX-608H
• Product Overview: LOX, 169-417aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 169-417 a.a.
• Description: LOX is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. Recombinant human LOX protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
• Molecular Mass: 31.4 kDa (272aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSDDPYNPY KYSDDNPYYN YYDTYERPRP GGRYRPGYGT GYFQYGLPDL VADPYYIQAS TYVQKMSMYN LRCAAEENCL ASTAYRADVR DYDHRVLLRF PQRVKNQGTS DFLPSRPRYS WEWHSCHQHY HSMDEFSHYD LLDANTQRRV AEGHKASFCL EDTSCDYGYH RRFACTAHTQ GLSPGCYDTY GADIDCQWID ITDVKPGNYI LKVSVNPSYL VPESDYTNNV VRCDIRYTGH HAYASGCTIS PY
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Publications:
  LOX, but not LOXL2, promotes bone metastasis formation and bone destruction in triple-negative breast cancer (2024)

Gene Information

• Gene Name: LOX lysyl oxidase [ Homo sapiens ]
• Official Symbol: LOX
• Synonyms: LOX; lysyl oxidase; protein-lysine 6-oxidase; MGC105112
• Gene ID: 4015
• mRNA Refseq: NM_001178102
• Protein Refseq: NP_001171573
• MIM: 153455
• UniProt ID: P28300
• Chromosome Location: 5q23.3-q31.2
• Function: copper ion binding; metal ion binding; oxidoreductase activity; protein-lysine 6-oxidase activity