Recombinant Human MAP3K3

• Cat.No.: MAP3K3-30192TH
• Product Overview: Recombinant full-length Human MEKK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal proprietary tag.

Specification

• Description: This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Biological activity: Specific activity: 20nmol/min/mg.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 OPR domain.Contains 1 protein kinase domain.

Gene Information

• Gene Name: MAP3K3 mitogen-activated protein kinase kinase kinase 3 [ Homo sapiens ]
• Official Symbol: MAP3K3
• Synonyms: MAP3K3; mitogen-activated protein kinase kinase kinase 3; MEKK3; MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3; MAPKKK3
• Gene ID: 4215
• mRNA Refseq: NM_002401
• Protein Refseq: NP_002392
• MIM: 602539
• Uniprot ID: Q99759
• Chromosome Location: 17q
• Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; GnRH signaling pathway, organism-specific biosystem; GnRH signaling pathway, conserved biosystem; HTLV-I infection, organism-specific biosystem
• Function: ATP binding; MAP kinase kinase kinase activity; metal ion binding; nucleotide binding; protein binding