Recombinant Human MAP4K5 protein, His&GST-tagged

• Cat.No.: MAP4K5-947H
• Product Overview: Recombinant Human MAP4K5 protein(NP_006566.2) (Met 1-Tyr 846) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-846 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM TCEP, pH 8.5.
• Molecular Mass: The recombinant human MAP4K5/GST chimera consists of 1083 amino acids and has a calculated molecular mass of 123 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 82 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MAP4K5 mitogen-activated protein kinase kinase kinase kinase 5 [ Homo sapiens ]
• Official Symbol: MAP4K5
• Synonyms: MAP4K5; mitogen-activated protein kinase kinase kinase kinase 5; GCKR; germinal center kinase related; KHS; KHS1; MEKKK 5; MEK kinase kinase 5; germinal center kinase-related; MAPK/ERK kinase kinase kinase 5; kinase homologous to SPS1/STE20; MAPKKKK5
• Gene ID: 11183
• mRNA Refseq: NM_006575
• Protein Refseq: NP_006566
• MIM: 604923
• UniProt ID: Q9Y4K4