Recombinant Human MAPK13, His-tagged

• Cat.No.: MAPK13-31379TH
• Product Overview: Recombinant full length Human SAPK4 with an N terminal His tag; 385 amino acids with tag, Predicted MWt 44.2 kDa.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.
• Protein length: 365 amino acids
• Conjugation: HIS
• Molecular Weight: 44.200kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.02% DTT
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMSLIR KKGFYKQDVNKTAWELPKTYVSPTH VGSGAYGSVCSAIDKRSGEKVAIKK LSRPFQSEIFAKRAYRELLLLKHMQ HENVIGLLDVFTPASSLRNFYDFYL VMPFMQTDLQKIMGMEFSEEKIQYL VYQMLKGLKYIHSAGVVHRDLKPGN LAVNEDCELKILDFGLARHADAEMT GYVVTRWYRAPEVILSWMHYNQTVD IWSVGCIMAEMLTGKTLFKGKDYLD QLTQILKVTGVPGTEFVQKLNDKAA KSYIQSLPQTPRKDFTQLFPRASPQ AADLLEKMLELDVDKRLTAAQALTH PFFEPFRDPEEETEAQQPFDDSLEH EKLTVDEWKQHIYKEIVNFSPIARK DSRRRSGMKL
• Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: MAPK13 mitogen-activated protein kinase 13 [ Homo sapiens ]
• Official Symbol: MAPK13
• Synonyms: MAPK13; mitogen-activated protein kinase 13; PRKM13; p38delta; SAPK4
• Gene ID: 5603
• mRNA Refseq: NM_002754
• Protein Refseq: NP_002745
• MIM: 602899
• Uniprot ID: O15264
• Chromosome Location: 6p21
• Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Cell-Cell communication, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem
• Function: ATP binding; MAP kinase activity; MAP kinase activity; nucleotide binding; protein binding