Recombinant Human MAPKAPK5, GST-His

• Cat.No.: MAPKAPK3-695H
• Product Overview: Recombinant, human MAPKAPK5 is fused at the N-terminus to a GST-His 6-thrombin cleavage site tag and expressed inS. frugiperda insect cellsusing a baculovirus expression system. MAPKAPK5 is a member of the serine/threonine kinase family that mediates stress-induced small heat shock protein 27 phosphorylation.

Specification

• Species: Human
• Source: S.frugiperda
• Tag: GST&His
• Description: MAP kinase-activated protein kinase 5 is an enzyme that in humans is encoded by the MAPKAPK5 gene. The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Form: Liquid.
• Formulation: In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
• Molar Mass: 84116 Da.
• Specific Activity: ≥2 U/mg protein.
• Unit Definition: One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to tetra(LRRWSLG) per min at 30°C using concentration of 0.1 μM ATP.
• Purity: ≥90% by SDS-PAGE.
• Storage: ≤ -70oC. Ship: Dry Ice Only.

Gene Information

• Gene Name: MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 [ Homo sapiens ]
• Synonyms:
  MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase; PRAK; EC 2.7.11.1
• Gene ID: 8550
• mRNA Refseq: NM_003668
• Protein Refseq: NP_003659
• MIM: 606723
• UniProt ID: Q8IW41
• Chromosome Location: 12q24.12-q24.13
• Pathway: MAPK signaling pathway
• Function: ATP binding; MAP kinase kinase activity; nucleotide binding; protein