Active Recombinant Full Length Human MAPKAPK5 Protein, GST-tagged

• Cat.No.: MAPKAPK5-8937HF
• Product Overview: Full-length human MAPKAPK5 [1-471(end) amino acids of accession number NP_003659.2] was co- expressed as N-terminal GST-fusion protein (81 kDa) with human His-tagged p38β [1-364(end) amino acids of accession number NP_002742.3] and human His-tagged MAP2K6 [1-334(end) amino acids of accession number NP_002749] using baculovirus expression system. GST-MAPKAPK5 was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-471
• Purity: 90%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase-activated protein kinase 5
• Official Symbol: MAPKAPK5
• Synonyms: MAPKAPK5;MAPKAP-K5;MK-5;MK5;PRAK
• Protein Refseq: NP_003659.2
• UniProt ID: Q8IW41