Recombinant Human MCL1, His-tagged

• Cat.No.: MCL1-781H
• Product Overview: Recombinant human MCL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
• Molecular Mass: 37.2 kDa (347aa), confirmed by MALDI-TOF
• Protein length: 1-327aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH PDSRRVARPP PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH VEDLEGG
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Publication:
  Mcl-1 Interacts with Akt to Promote Lung Cancer Progression (2019)

Gene Information

• Gene Name: MCL1 myeloid cell leukemia 1 [ Homo sapiens (human) ]
• Official Symbol: MCL1
• Synonyms: MCL1; myeloid cell leukemia sequence 1 (BCL2-related); induced myeloid leukemia cell differentiation protein Mcl-1; BCL2L3; Mcl 1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; TM; EAT; MCL1L; MCL1S; Mcl-1; MCL1-ES; bcl2-L-3; mcl1/EAT; MGC1839; MGC104264
• Gene ID: 4170
• mRNA Refseq: NM_021960
• Protein Refseq: NP_068779
• MIM: 159552
• UniProt ID: Q07820
• Chromosome Location: 1q21
• Pathway: Apoptosis Modulation and Signaling; E2F transcription factor network; IL-7 Signaling Pathway
• Function: BH3 domain binding; protein channel activity; protein heterodimerization activity

Reviews

06/20/2022

Another advantage of using MCL1 protein in trials is its potential as a therapeutic agent.

01/16/2022

The manufacturer's support and expertise further enhance the utility of MCL1 in my experimental work, ensuring reliable and high-quality results.

03/23/2016

This collaborative partnership greatly contributes to the success of my research projects and facilitates meaningful contributions to the scientific community.

Q&As

Can targeting MCL1 have implications beyond cancer therapy?

Yes, MCL1 modulation may have therapeutic implications in other diseases, such as neurodegenerative disorders, where apoptosis is a contributing factor.

Are there any diagnostic tools or biomarkers that can assess MCL1 expression in cancer patients?

Immunohistochemistry and molecular assays can be used to measure MCL1 expression levels, providing insights into the prognosis and potential treatment strategies.

How does the understanding of MCL1 function contribute to personalized medicine in cancer treatment?

Understanding MCL1 expression allows for the identification of patients who may benefit from targeted therapies, contributing to personalized treatment strategies.

Are there any ongoing clinical trials exploring MCL1 inhibitors as a therapeutic approach?

Yes, there are several clinical trials investigating the use of MCL1 inhibitors in different cancer types to assess their safety and efficacy.

How does the MCL1 protein interact with other proteins in the Bcl-2 family?

MCL1 interacts with pro-apoptotic and anti-apoptotic proteins in the Bcl-2 family, influencing the balance between cell survival and death.