Recombinant Human MGMT Protein, His-tagged MGMT-004H

Recombinant Human MGMT Protein, His-tagged


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Recombinant Human MGMT Protein, His-tagged

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Cat.No. : MGMT-004H
Product Overview : Recombinant Human MGMT protein with a N-terminal His-tag was expressed in E. coli
Description : Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 26.8 kDa
Purity : ≥50% estimated by SDS-PAGE
Stability : ≥ 6 months
Storage : -80 centigrade
Storage Buffer : 50 mM Tris, pH 8.0, 150 mM sodium chloride, 10% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
SDS-PAGE analysis of MGMT. :

Lane 1: MGMT (0 µg)
Lane 2: MGMT (0.5 µg)
Lane 3: MGMT (1.5 µg)
Lane 4: MGMT (4.5 µg)
Lane 5: MGMT (10 µg)
Lane 6: Unmethylated DNA oligo

ATPase Activity of MGMT :

Lane 1: MW Markers
Lane 2: MGMT (1 µg)
Lane 3: MGMT (2 µg)
Lane 4: MGMT (4 µg)

Gene Name : MGMT O-6-methylguanine-DNA methyltransferase[ Homo sapiens (human) ]
Official Symbol : MGMT
Synonyms : MGMT; O-6-methylguanine-DNA methyltransferase; Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; methylguanine-DNA methyltransferase; EC
Gene ID : 4255
mRNA Refseq : NM_002412
Protein Refseq : NP_002403
MIM : 156569
UniProt ID : P16455

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