Recombinant Human Mitogen-activated Protein Kinase-Activated Protein Kinase 5, GST-tagged

• Cat.No.: MAPKAPK5-1056H
• Product Overview: Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines. MAPKAPK5 is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta (1). MAPKAPK5 is activated in HeLa cells in response to cellular stress and proinflammatory cytokines. MAPKAPK5 activity is regulated by p38-alpha and p38-beta both in vitro and in vivo, and thr-182 is the regulatory phosphorylation site of MAPKAPK5 (2). In vitro, MAPKAPK5 kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 79 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 172 nmol/min/mg
• Purity: > 80%
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: MAPK signaling pathway

Gene Information

• Gene Name: MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 [ Homo sapiens ]
• Official Symbol: MAPKAPK5
• Synonyms: MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase; PRAK; EC 2.7.11.1
• Gene ID: 8550
• mRNA Refseq: NM_003668
• Protein Refseq: NP_003659
• MIM: 606723
• UniProt ID: Q8IW41
• Chromosome Location: 12q24.12-q24.13
• Function: ATP binding; MAP kinase kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity