Recombinant Human MMP1 protein, His-tagged

Cat.No. : MMP1-809H
Product Overview : Recombinant Human MMP1 (Phe20-Asn469) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
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Description : Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.
Matrix metalloproteinase-1 (MMP-1) is also known as interstitial collagenase and fibroblast collagenase. MMP­1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP­1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L­Selectin, pro­TNF, IL­1β, IGF­BP3, IGF­BP5, pro MMP­2 and pro MMP­9.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Phe20
Form : Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, 10 mM CaCl2, pH6.0 with 0.05% Brij-35, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 52.7 kDa. The protein migrates as 57-64 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Phe20-Asn469
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
FACS: Human MMP-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Gene Name : MMP1
Official Symbol : MMP1
Synonyms : MMP1; matrix metallopeptidase 1 (interstitial collagenase); CLG, matrix metalloproteinase 1 (interstitial collagenase); interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; CLG; CLGN
Gene ID : 4312
mRNA Refseq : NM_001145938
Protein Refseq : NP_001139410
MIM : 120353
UniProt ID : P03956

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does MMP1 relate to wound healing? 02/09/2023

MMP1 is involved in the remodeling of tissue during wound healing by facilitating the breakdown of damaged extracellular matrix components.

Are there therapeutic implications of targeting MMP1 in cancer treatment? 11/11/2022

Inhibiting MMP1 activity is being explored as a potential therapeutic strategy to impede cancer progression and metastasis.

How does MMP1 contribute to tissue remodeling? 05/07/2020

MMP1 plays a key role in tissue remodeling by degrading collagen, a major component of the extracellular matrix, which is essential for processes like wound healing.

In what diseases is MMP1 overexpressed? 08/21/2017

MMP1 overexpression is associated with various diseases, including rheumatoid arthritis, atherosclerosis, and certain types of cancer.

Can MMP1 be a biomarker for prognosis in cancer patients? 02/18/2017

High MMP1 levels have been correlated with poor prognosis in certain cancer types, making it a potential biomarker for predicting disease outcomes.

Customer Reviews (3)

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Reviews
08/26/2021

    The manufacturer can offer technical support and expertise to guide researchers through the experimental process.

    11/09/2020

      The manufacturer can play a vital role in supporting and optimizing the research process.

      07/20/2020

        This guarantees that the protein is reliable, consistent, and suitable for experiments, reducing variability and enhancing the reproducibility of findings.

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