Recombinant Human MMP1 protein, His-tagged

• Cat.No.: MMP1-809H
• Product Overview: Recombinant Human MMP1 (Phe20-Asn469) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.
  Matrix metalloproteinase-1 (MMP-1) is also known as interstitial collagenase and fibroblast collagenase. MMP­1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP­1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L­Selectin, pro­TNF, IL­1β, IGF­BP3, IGF­BP5, pro MMP­2 and pro MMP­9.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Phe20
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, 10 mM CaCl2, pH6.0 with 0.05% Brij-35, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 52.7 kDa. The protein migrates as 57-64 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Phe20-Asn469
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: MMP1
• Official Symbol: MMP1
• Synonyms: MMP1; matrix metallopeptidase 1 (interstitial collagenase); CLG, matrix metalloproteinase 1 (interstitial collagenase); interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; CLG; CLGN
• Gene ID: 4312
• mRNA Refseq: NM_001145938
• Protein Refseq: NP_001139410
• MIM: 120353
• UniProt ID: P03956

Reviews

08/26/2021

The manufacturer can offer technical support and expertise to guide researchers through the experimental process.

11/09/2020

The manufacturer can play a vital role in supporting and optimizing the research process.

07/20/2020

This guarantees that the protein is reliable, consistent, and suitable for experiments, reducing variability and enhancing the reproducibility of findings.

Q&As

How does MMP1 relate to wound healing?

MMP1 is involved in the remodeling of tissue during wound healing by facilitating the breakdown of damaged extracellular matrix components.

Are there therapeutic implications of targeting MMP1 in cancer treatment?

Inhibiting MMP1 activity is being explored as a potential therapeutic strategy to impede cancer progression and metastasis.

How does MMP1 contribute to tissue remodeling?

MMP1 plays a key role in tissue remodeling by degrading collagen, a major component of the extracellular matrix, which is essential for processes like wound healing.

In what diseases is MMP1 overexpressed?

MMP1 overexpression is associated with various diseases, including rheumatoid arthritis, atherosclerosis, and certain types of cancer.

Can MMP1 be a biomarker for prognosis in cancer patients?

High MMP1 levels have been correlated with poor prognosis in certain cancer types, making it a potential biomarker for predicting disease outcomes.