Recombinant Human MRPL18 Protein, GST-tagged

• Cat.No.: MRPL18-5562H
• Product Overview: Human MRPL18 full-length ORF ( NP_054880.2, 1 a.a. - 180 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 47 kDa
• AA Sequence: MALRSRFWGLFSVCRNPGCRFAALS TSSEPAAKPEVDPVENEAVAPEFTN RNPRNLELLSVARKERGWRTVFPSR EFWHRLRVIRTQHHVEALVEHQNGK VVVSASTREWAIKKHLYSTRNVVAC ESIGRVLAQRCLEAGINFMVYQPTP WEAASDSMKRLQSAMTEGGVVLREP QRIYE
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MRPL18 mitochondrial ribosomal protein L18 [ Homo sapiens ]
• Official Symbol: MRPL18
• Synonyms: MRPL18; mitochondrial ribosomal protein L18; 39S ribosomal protein L18, mitochondrial; HSPC071; L18mt; MRP-L18
• Gene ID: 29074
• mRNA Refseq: NM_014161
• Protein Refseq: NP_054880
• MIM: 611831
• UniProt ID: Q9H0U6