Recombinant Human MRPL42 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MRPL42-3365H
• Product Overview: MRPL42 MS Standard C13 and N15-labeled recombinant protein (NP_054769) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 16.7 kDa
• AA Sequence: MAVAAVKWVMSKRTILKHLFPVQNG ALYCVCHKSTYSPLPDDYNCNVELA LTSDGRTIVCYHPSVDIPYEHTKPI PRPDPVHNNEETHDQVLKTRLEEKV EHLEEGPMIEQLSKMFFTTKHRWYP HGRYHRCRKNLNPPKDRTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MRPL42 mitochondrial ribosomal protein L42 [ Homo sapiens (human) ]
• Official Symbol: MRPL42
• Synonyms: MRPL42; mitochondrial ribosomal protein L42; RPML31; MRP-L31; 39S ribosomal protein L42, mitochondrial; 28S ribosomal protein S32, mitochondrial; 39S ribosomal protein L31, mitochondrial; mitochondrial large ribosomal subunit protein mL42
• Gene ID: 28977
• mRNA Refseq: NM_014050
• Protein Refseq: NP_054769
• MIM: 611847
• UniProt ID: Q9Y6G3