Active Recombinant Human MS4A1 protein, His-tagged
Cat.No. : | MS4A1-48392H |
Product Overview : | Recombinant Human MS4A1 Protein (Met1-Pro297) is produced by Baculovirus-Insect cells expression system. This protein carries a polyhistidine tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Description : | B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. The protein has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. CD20 / MS4A1 is the target of the monoclonal antibodies (mAb) rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. |
Source : | Baculovirus-Insect cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Met 1 |
Form : | Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH 7.5 with Glycerol as protectant. |
Bio-activity : | Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, insect cell with a linear range of 1-16 ng/mL (in presence of DDM and CHS) (QC tested). Immobilized Human CD20 Full Length, His Tag, insect cell at 2 μg/mL (100 μL/well) on Nickel Coated Plate, can bind Rituximab with a linear range of 0.313-1.25 μg/mL (Routinely tested). Immobilized Ofatumumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, insect cell with a linear range of 2-31 ng/mL (in presence of DDM and CHS) (Routinely tested). |
Molecular Mass : | The protein has a calculated MW of 35.2 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Met1-Pro297 |
Endotoxin : | < 1.0 EU per μg by the LAL method. |
Purity : | > 80% as determined by SDS-PAGE. |
Storage : | Please avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. |
Concentration : | Contact us for detailes. |
Gene Name : | MS4A1 membrane-spanning 4-domains, subfamily A, member 1 [ Homo sapiens ] |
Official Symbol : | MS4A1 |
Synonyms : | MS4A1; CD20; B-lymphocyte antigen CD20; B1; Bp35; MS4A2; CD20 antigen; CD20 receptor; S7; CVID5; LEU-16; MGC3969; |
Gene ID : | 931 |
mRNA Refseq : | NM_021950 |
Protein Refseq : | NP_068769 |
MIM : | 112210 |
UniProt ID : | P11836 |
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◆ Lysates | ||
MS4A1-1848FCL | Recombinant Ferret MS4A1 cell lysate | +Inquiry |
MS4A1-1542HCL | Recombinant Human MS4A1 cell lysate | +Inquiry |
MS4A1-819CCL | Recombinant Cynomolgus MS4A1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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