||Recombinant human MSLN is expressed as a 317-amino acid protein consisting of Glu296 - Ser592 region of MSLN (UniProt accession #Q13421, isoform 2) and a C-terminal His-tag. It contains 4 potential N-linked glycosylation sites.
||Binds to CA125/MUC16 and anti-MSLN monoclonal antibody, human IgG1 with high affinity KD < 5 nM as measured by ELISA.
||Calculated molecular mass (kDa): 35.9; Estimated by SDS-PAGE under reducing condition (kDa): 48-50
||<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
||>90% judged by SDS-PAGE under reducing condition.
||The product is shipped at 4 centigrade. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
||Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
||FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.