Recombinant Human MSLN Protein (Glu 296 - Gly 580), His-tagged, PE-Labeled

• Cat.No.: MSLN-318H
• Product Overview: Recombinant Human MSLN Protein, His-tagged, PE-Labeled is produced via conjugation of PE to Human Mesothelin (296-580) under optimal conditions. Human Mesothelin (296-580) is expressed in HEK293 cells containing AA Glu 296 - Gly 580 (Accession # AAH09272). Predicted N-terminus: Glu 296. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. Conjugate (PE): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 575 nm.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 10 mM Tris, 150 mM NaCl, 0.1% BSA, pH8.2. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: 33.0 kDa
• Protein length: Glu 296 - Gly 580
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421