Active Recombinant Human MSLN Protein, His-tagged, Site-specific PE-Labeled

• Cat.No.: MSLN-4682H
• Product Overview: Site-specific PE-Labeled Active Recombinant Human MSLN Protein(AAH09272)(Glu 296 - Gly 580), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Glu 296 - Gly 580
• Conjugation/Label: Site-specific PE
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
• Bio-activity: 1e6 of the anti-MSLN CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Mesothelin (296-580) Protein, His Tag and negative control protein respectively, PE signal was used to evaluate the binding activity.
• Molecular Mass: The protein has a calculated MW of 35.9 kDa.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please protect from light and avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 6 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421

Reviews

05/22/2022

Very satisfied, great product.

03/06/2022

Product is high-quality.

09/30/2021

Excellent for experiments, reliable.

Q&As

What is the primary role of MSLN in normal tissues?

MSLN is normally expressed in mesothelial cells, playing a role in cell adhesion.

How is MSLN expression altered in cancerous cells?

In cancer, MSLN expression is significantly upregulated.

In which types of cancer is MSLN overexpressed?

MSLN is overexpressed in mesothelioma, ovarian, and pancreatic cancers.

Are there any therapeutic strategies targeting MSLN in cancer treatment?

Therapies targeting MSLN include antibody-drug conjugates and CAR-T cell therapies.

How can MSLN levels be detected and measured in clinical settings?

MSLN levels can be assessed through immunohistochemistry and serum biomarker tests.

What is the prognostic significance of MSLN overexpression in cancer patients?

MSLN overexpression is often associated with poor prognosis in cancer patients.

How does MSLN contribute to cancer progression and metastasis?

MSLN promotes tumor cell proliferation, invasion, and resistance to apoptosis.