Recombinant Human MTF1 protein, His & T7-tagged

• Cat.No.: MTF1-7962H
• Product Overview: Recombinant Human MTF1 aa. (Gly2~His164 (Accession # Q14872)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE).
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly2~His164 (Accession # Q14872)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: MTF1 metal regulatory transcription factor 1 [ Homo sapiens (human) ]
• Official Symbol: MTF1
• Synonyms: MTF1; metal regulatory transcription factor 1; ZRF; MTF-1; MRE-binding transcription factor; MRE-binding transcription factor-1; metal-responsive transcription factor 1; transcription factor MTF-1; zinc regulatory factor
• Gene ID: 4520
• mRNA Refseq: NM_005955.2
• Protein Refseq: NP_005946.2
• UniProt ID: Q14872