Recombinant Human MTHFD2, T7-tagged

• Cat.No.: MTHFD2-393H
• Product Overview: Recombinant Human MTHFD2 was expressed inE.Coliusing an N-terminal T7 tag. MW=39.56 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Description: This is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD.
• Sequence: 1-344.
• Purity: ~95%.
• Specific Activity: n/aH.
• Applications: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
• Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.
• Storage: Store at -70?C. As with any protein, exposing MTHFD2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS PAGE Analysis of MTHFD2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.

Gene Information

• Gene Name: MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase [ Homo sapiens ]
• Synonyms: MTHFD2; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase; NMDMC; methylenetetrahydrofolate dehydrogenase 2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; EC 1.5.1.15
• Gene ID: 10797
• mRNA Refseq: NM_006636
• Protein Refseq: NP_006627
• MIM: 604887
• UniProt ID: P13995
• Chromosome Location: 2p13.1
• Pathway: Glyoxylate and dicarboxylate metabolism; Metabolic pathways; One carbon pool by folate
• Function: binding; hydrolase activity; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; oxidoreductase activity; phosphate binding