Recombinant Human NAGPA Protein, MYC/DDK-tagged

• Cat.No.: NAGPA-189H
• Product Overview: Recombinant human NAGPA protein, fused to MYC/DDK-tag at C-terminal, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015].
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
• Molecular Mass: 53.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase [ Homo sapiens ]
• Official Symbol: NAGPA
• Synonyms: APAA; UCE
• Gene ID: 51172
• mRNA Refseq: NM_016256.3
• Protein Refseq: NP_057340.2
• MIM: 607985
• UniProt ID: Q9UK23