Recombinant Human NCALD protein, His-tagged

• Cat.No.: NCALD-325H
• Product Overview: Recombinant Human NCALD protein (Gly2-Phe193), fused to His tag at N-terminus, was expressed in E.coli.

Specification

• Description: Neurocalcin-delta (NCALD) is a member of the recoverin family, which contains four EF-hand domains. Neurocalcin is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family of proteins. Neurocalcin is expressed in mammalian brains and possesses a Ca2+/myristoyl switch. As for NCALD, it may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. NCALD binds three calcium ions.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.1 kDa. The protein migrates as 23 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: 192
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: NCALD
• Official Symbol: NCALD
• Synonyms: NCALD; neurocalcin delta; neurocalcin-delta; MGC33870; MGC74858
• Gene ID: 83988
• mRNA Refseq: NM_001040624
• Protein Refseq: NP_001035714
• MIM: 606722
• UniProt ID: P61601