Recombinant Human NEDD4L Protein, MYC/DDK-tagged, C13 and N15-labeled

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Recombinant Human NEDD4L Protein, MYC/DDK-tagged, C13 and N15-labeled

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Cat.No. : NEDD4L-034H
Product Overview : NEDD4L MS Standard C13 and N15-labeled recombinant protein (NP_001138436) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 98.2 kDa
AA Sequence : MERPYTFKDF LLRPRSHKSR VKGFLRLKMA YMPKNGGQDE ENSDQRDDME HGWEVVDSND SASQHQEELP PPPLPPGWEE KVDNLGRTYY VNHNNRTTQW HRPSLMDVSS ESDNNIRQIN QEAAHRRFRS RRHISEDLEP EPSEGGDVPE PWETISEEVN IAGDSLGLAL PPPPASPGSR TSPQELSEEL SRRLQITPDS NGEQFSSLIQ REPSSRLRSC SVTDAVAEQG HLPPPSAPAG RARSSTVTGG EEPTPSVAYV HTTPGLPSGW EERKDAKGRT YYVNHNNRTT TWTRPIMQLA EDGASGSATN SNNHLIEPQI RRPRSLSSPT VTLSAPLEGA KDSPVRRAVK DTLSNPQSPQ PSPYNSPKPQ HKVTQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSKTS LNPNDLGPLP PGWEERIHLD GRTFYIDHNS KITQWEDPRL QNPAITGPAV PYSREFKQKY DYFRKKLKKP ADIPNRFEMK LHRNNIFEES YRRIMSVKRP DVLKARLWIE FESEKGLDYG GVAREWFFLL SKEMFNPYYG LFEYSATDNY TLQINPNSGL CNEDHLSYFT FIGRVAGLAV FHGKLLDGFF IRPFYKMMLG KQITLNDMES VDSEYYNSLK WILENDPTEL DLMFCIDEEN FGQTYQVDLK PNGSEIMVTN ENKREYIDLV IQWRFVNRVQ KQMNAFLEGF TELLPIDLIK IFDENELELL MCGLGDVDVN DWRQHSIYKN GYCPNHPVIQ WFWKAVLLMD AEKRIRLLQF VTGTSRVPMN GFAELYGSNG PQLFTIEQWG SPEKLPRAHT CFNRLDLPPY ETFEDLREKL LMAVENAQGF EGVDTRTRPL EQKLISEEDL AANDILDYKD DDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : NEDD4L NEDD4 like E3 ubiquitin protein ligase [ Homo sapiens (human) ]
Official Symbol : NEDD4L
Synonyms : NEDD4L; NEDD4 like E3 ubiquitin protein ligase; hNEDD4-2; NEDD4-2; NEDD4.2; PVNH7; RSP5; E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase; ubiquitin-protein ligase Rsp5; EC 2.3.2.26
Gene ID : 23327
mRNA Refseq : NM_001144964
Protein Refseq : NP_001138436
MIM : 606384
UniProt ID : Q96PU5

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