Recombinant Human NEFL protein, MYC/DDK-tagged

• Cat.No.: NEFL-13H
• Product Overview: Recombinant Human NEFL fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 61.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Quality Control Test: 54.5 kDa
• Notes: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: NEFL neurofilament, light polypeptide [ Homo sapiens ]
• Official Symbol: NEFL
• Synonyms: NEFL; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament light polypeptide; CMT1F; CMT2E; NF68; NFL; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; light molecular weight neurofilament protein; NF-L; FLJ53642
• Gene ID: 4747
• mRNA Refseq: NM_006158
• Protein Refseq: NP_006149
• MIM: 162280
• UniProt ID: P07196
• Chromosome Location: 8p21.2
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; CREB phosphorylation through the activation of CaMKII, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Neuronal System, organism-specific biosystem; Neurotransmitter Receptor Binding And Downstream Transmission In ThePostsynaptic Cell, organism-specific biosystem
• Function: identical protein binding; protein C-terminus binding; protein binding; structural constituent of cytoskeleton