Recombinant Human NFKB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human NFKB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : NFKB1-5277H
Product Overview : NFKB1 MS Standard C13 and N15-labeled recombinant protein (NP_003989) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 105.2 kDa
AA Sequence : MAEDDPYLGR PEQMFHLDPS LTHTIFNPEV FQPQMALPTA DGPYLQILEQ PKQRGFRFRY VCEGPSHGGL PGASSEKNKK SYPQVKICNY VGPAKVIVQL VTNGKNIHLH AHSLVGKHCE DGICTVTAGP KDMVVGFANL GILHVTKKKV FETLEARMTE ACIRGYNPGL LVHPDLAYLQ AEGGGDRQLG DREKELIRQA ALQQTKEMDL SVVRLMFTAF LPDSTGSFTR RLEPVVSDAI YDSKAPNASN LKIVRMDRTA GCVTGGEEIY LLCDKVQKDD IQIRFYEEEE NGGVWEGFGD FSPTDVHRQF AIVFKTPKYK DINITKPASV FVQLRRKSDL ETSEPKPFLY YPEIKDKEEV QRKRQKLMPN FSDSFGGGSG AGAGGGGMFG SGGGGGGTGS TGPGYSFPHY GFPTYGGITF HPGTTKSNAG MKHGTMDTES KKDPEGCDKS DDKNTVNLFG KVIETTEQDQ EPSEATVGNG EVTLTYATGT KEESAGVQDN LFLEKAMQLA KRHANALFDY AVTGDVKMLL AVQRHLTAVQ DENGDSVLHL AIIHLHSQLV RDLLEVTSGL ISDDIINMRN DLYQTPLHLA VITKQEDVVE DLLRAGADLS LLDRLGNSVL HLAAKEGHDK VLSILLKHKK AALLLDHPNG DGLNAIHLAM MSNSLPCLLL LVAAGADVNA QEQKSGRTAL HLAVEHDNIS LAGCLLLEGD AHVDSTTYDG TTPLHIAAGR GSTRLAALLK AAGADPLVEN FEPLYDLDDS WENAGEDEGV VPGTTPLDMA TSWQVFDILN GKPYEPEFTS DDLLAQGDMK QLAEDVKLQL YKLLEIPDPD KNWATLAQKL GLGILNNAFR LSPAPSKTLM DNYEVSGGTV RELVEALRQM GYTEAIEVIQ AASSPVKTTS QAHSLPLSPA STRQQIDELR DSDSVCDSGV ETSFRKLSFT ESLTSGASLL TLNKMPHDYG QEGPLEGKIT RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 [ Homo sapiens (human) ]
Official Symbol : NFKB1
Synonyms : NFKB1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor NF-kappa-B p105 subunit; KBF1; NF kappaB; NF kB1; NFkappaB; NFKB p50; p50; p105; NF-kappabeta; DNA binding factor KBF1; DNA-binding factor KBF1; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit; EBP-1; NF-kB1; NFKB-p50; NF-kappaB; NFKB-p105; NF-kappa-B; MGC54151; DKFZp686C01211;
Gene ID : 4790
mRNA Refseq : NM_003998
Protein Refseq : NP_003989
MIM : 164011
UniProt ID : P19838

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