Recombinant Human NME1 protein NME1-192H

Recombinant Human NME1 protein

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Recombinant Human NME1 protein

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Cat.No. : NME1-192H
Product Overview : Recombinant human NME1 was expressed in E.coli and purified by using conventional chromatography techniques.
Description : Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.
Source : E.coli
Species : Human
Form : Liquid. In 20mM Tris-HCl buffer (pH7.5) containing 1mM DTT, 10% glycerol
Bio-activity : Specific activity is > 1,200 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Molecular Mass : 17.1 kDa (152aa), confirmed by MALDI-TOF
AA Sequence : MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVMLGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE
Endotoxin : < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity : > 90% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : NME1 non-metastatic cells 1, protein (NM23A) expressed in [ Homo sapiens ]
Official Symbol : NME1
Synonyms : NME1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; NM23; NM23 H1; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; NB; AWD; NBS; GAAD; NDKA; NDPKA; NDPK-A; NM23-H1;
Gene ID : 4830
mRNA Refseq : NM_000269
Protein Refseq : NP_000260
MIM : 156490
UniProt ID : P15531
Chromosome Location : 17q21.33
Pathway : Arf6 downstream pathway, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Purine metabolism, organism-specific biosystem;
Function : ATP binding; GTP binding; deoxyribonuclease activity; gamma-tubulin binding; identical protein binding; kinase activity; magnesium ion binding; nucleoside diphosphate kinase activity; nucleotide binding; protein binding; transferase activity;

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