Active Recombinant Human NME1 protein

• Cat.No.: NME1-192H
• Product Overview: Recombinant human NME1 was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.
• Source: E.coli
• Species: Human
• Form: Liquid. In 20mM Tris-HCl buffer (pH7.5) containing 1mM DTT, 10% glycerol
• Bio-activity: Specific activity is > 1,200 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
• Molecular Mass: 17.1 kDa (152aa), confirmed by MALDI-TOF
• AA Sequence: MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVMLGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NME1 non-metastatic cells 1, protein (NM23A) expressed in [ Homo sapiens ]
• Official Symbol: NME1
• Synonyms: NME1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; NM23; NM23 H1; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; NB; AWD; NBS; GAAD; NDKA; NDPKA; NDPK-A; NM23-H1
• Gene ID: 4830
• mRNA Refseq: NM_000269
• Protein Refseq: NP_000260
• MIM: 156490
• UniProt ID: P15531
• Chromosome Location: 17q21.33
• Pathway: Arf6 downstream pathway, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Purine metabolism, organism-specific biosystem
• Function: ATP binding; GTP binding; deoxyribonuclease activity; gamma-tubulin binding; identical protein binding; kinase activity; magnesium ion binding; nucleoside diphosphate kinase activity; nucleotide binding; protein binding; transferase activity

Reviews

04/02/2022

Critical expertise in protein engineering, unmatched in quality.

10/28/2021

Reliable protein crystallization services, consistently yields exceptional results.

12/23/2018

Efficient protein purification, consistent yields, cost-effective for research.

Q&As

What role does NME1 play in DNA repair and the maintenance of genomic stability?

NME1 plays a role in DNA repair, contributing to genomic stability.

How does NME1 influence signal transduction in response to cellular stress?

NME1 modulates cellular responses to stress, affecting signal transduction pathways.

What is the impact of NME1 on apoptosis and cell survival pathways?

NME1 influences apoptosis and cell survival, impacting cell fate decisions.

How do alterations in NME1 expression or activity affect drug resistance in cancer therapy?

Changes in NME1 can impact drug resistance in cancer, affecting treatment efficacy.

What are the mechanisms by which NME1 modulates the immune response, especially in tumor immunity?

NME1 regulates the immune response, particularly in tumor immunity.

How does NME1 contribute to the metastatic potential of cancer cells?

NME1 affects the metastatic behavior of cancer cells, influencing their spread.

How does NME1 regulate nucleotide metabolism and cellular energy balance?

NME1 is involved in nucleotide metabolism, crucial for maintaining cellular energy balance.