Active Recombinant Human NME3 protein, His-tagged

• Cat.No.: NME3-202H
• Product Overview: Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.1M NaCl, 2mM DTT
• Bio-activity: Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
• Molecular Mass: 19.1 kDa (169aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPVVAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NME3 non-metastatic cells 3, protein expressed in [ Homo sapiens ]
• Official Symbol: NME3
• Synonyms: NME3; non-metastatic cells 3, protein expressed in; nucleoside diphosphate kinase 3; DR nm23; NM23 H3; NDK 3; NDP kinase 3; NDP kinase C; nucleoside diphosphate kinase C; NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2; KIAA0516
• Gene ID: 4832
• mRNA Refseq: NM_002513
• Protein Refseq: NP_002504
• MIM: 601817
• UniProt ID: Q13232
• Chromosome Location: 16q13.3
• Pathway: Metabolic pathways, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem; Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP, organism-specific biosystem
• Function: ATP binding; kinase activity; metal ion binding; nucleoside diphosphate kinase activity; nucleotide binding; transferase activity