Recombinant Human NOTCH1 Protein, His-tagged

• Cat.No.: NOTCH1-2498H
• Product Overview: Recombinant Human NOTCH1 Protein (Glu1949-Tyr2209) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.7 kDa
  Accurate Molecular Mass: 36 kDa
• Protein length: Glu1949-Tyr2209
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: NOTCH1 notch receptor 1 [ Homo sapiens (human) ]
• Official Symbol: NOTCH1
• Synonyms: NOTCH1; notch receptor 1; hN1; AOS5; TAN1; AOVD1; neurogenic locus notch homolog protein 1
  Notch homolog 1, translocation-associated
  notch 1
  translocation-associated notch protein TAN-1
• Gene ID: 4851
• mRNA Refseq: NM_017617
• Protein Refseq: NP_060087
• MIM: 190198
• UniProt ID: P46531