Active Recombinant Human NT5E protein, hFc-tagged, Low Endotoxin

• Cat.No.: NT5E-580H
• Product Overview: Recombinant Human NT5E protein(P21589-1)(Trp27-Lys547), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 27-547 aa
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD73, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
• Official Symbol: NT5E
• Synonyms: NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73), NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT
• Gene ID: 4907
• mRNA Refseq: NM_001204813
• Protein Refseq: NP_001191742
• MIM: 129190
• UniProt ID: P21589