Recombinant Human PAFAH2 Protein, His-tagged PAFAH2-19H

Recombinant Human PAFAH2 Protein, His-tagged

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Recombinant Human PAFAH2 Protein, His-tagged

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Cat.No. : PAFAH2-19H
Product Overview : Recombinant human PAFAH2 protein (2-392) with a N-terminal His-tag was expressed in E. coli.
Description : This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES, pH 8.0, 150 mM sodium chloride, and 20% glycerol
Molecular Mass : 46.1 kDa
Protein length : 2-392
Unit Definition : One unit is defined as the amount required to produce 1 nmol of TNB per minute at 37 centigrade in 50 mM HEPES, pH 7.4, containing 150 mM sodium chloride, 5 mM CHAPS, 1 mM EDTA, 5% glycerol, and 300 µM 2-thio-PAF.
Applications : Enzyme Activity, WB, IP
Stability : ≥ 1 year
Storage : Store at -80 centigrade
SDS-PAGE of PAFAH2 :

Lane 1: MW Markers, Lane 2: PAF-AH2 (2 µg), Lane 3: PAF-AH2 (4 µg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

PAF-AH2 Activity Assay

Gene Name : PAFAH2 platelet activating factor acetylhydrolase 2 [ Homo sapiens (human) ]
Official Symbol : PAFAH2
Synonyms : PAFAH2; platelet activating factor acetylhydrolase 2; HSD-PLA2; platelet-activating factor acetylhydrolase 2, cytoplasmic; SD-PLA2; platelet-activating factor acetylhydrolase 2, 40kDa; serine-dependent phospholipase A2
Gene ID : 5051
mRNA Refseq : NM_000437
Protein Refseq : NP_000428
MIM : 602344
UniProt ID : Q99487

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