Recombinant Human PAFAH2 Protein, His-tagged


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Recombinant Human PAFAH2 Protein, His-tagged

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Cat.No. : PAFAH2-19H
Product Overview : Recombinant human PAFAH2 protein (2-392) with a N-terminal His-tag was expressed in E. coli.
Description : This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES, pH 8.0, 150 mM sodium chloride, and 20% glycerol
Molecular Mass : 46.1 kDa
Protein length : 2-392
Unit Definition : One unit is defined as the amount required to produce 1 nmol of TNB per minute at 37 centigrade in 50 mM HEPES, pH 7.4, containing 150 mM sodium chloride, 5 mM CHAPS, 1 mM EDTA, 5% glycerol, and 300 µM 2-thio-PAF.
Applications : Enzyme Activity, WB, IP
Stability : ≥ 1 year
Storage : Store at -80 centigrade

Lane 1: MW Markers, Lane 2: PAF-AH2 (2 µg), Lane 3: PAF-AH2 (4 µg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

PAF-AH2 Activity Assay

Gene Name : PAFAH2 platelet activating factor acetylhydrolase 2 [ Homo sapiens (human) ]
Official Symbol : PAFAH2
Synonyms : PAFAH2; platelet activating factor acetylhydrolase 2; HSD-PLA2; platelet-activating factor acetylhydrolase 2, cytoplasmic; SD-PLA2; platelet-activating factor acetylhydrolase 2, 40kDa; serine-dependent phospholipase A2
Gene ID : 5051
mRNA Refseq : NM_000437
Protein Refseq : NP_000428
MIM : 602344
UniProt ID : Q99487
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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