Cat.No. : |
PAOX-5304H |
Product Overview : |
PAOX MS Standard C13 and N15-labeled recombinant protein (NP_997011) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
Description : |
Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
51.8 kDa |
AA Sequence : |
MESTGSVGEA PGGPRVLVVG GGIAGLGAAQ RLCGHSAFPH LRVLEATARA GGRIRSERCF GGVVEVGAHW IHGPSRGNPV FQLAAEYGLL GEKELSQENQ LVETGGHVGL PSVSYASSGA SVSLQLVAEM ATLFYGLIDQ TREFLHAAET PVPSVGEYLK KEIGQHVAGW TEDEETRKLK LAVLNSFFNL ECCVSGTHSM DLVALAPFGE YTVLPGLDCT FSKGYQGLTN CMMAALPEDT VVFEKPVKTI HWNGSFQEAA FPGETFPVSV ECEDGDRFPA HHVIVTVPLG FLREHLDTFF DPPLPAEKAE AIRKIGFGTN NKIFLEFEEP FWEPDCQLIQ LVWEDTSPLE DAAPELQDAW FRKLIGFVVL PAFASVHVLC GFIAGLESEF METLSDEEVL LCLTQVLRRV TAPDPVCGGS HTSHVLLHDA RGSAVGMEGG RPPPQSVGPA GAAAQAQALA GPSLLCSTRV GGRLGPSFLL TDFSLATRTR PLEQKLISEE DLAANDILDY KDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
SDS-PAGE : |
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