Species : |
Human |
Source : |
E.coli |
Tag : |
GST&His |
Protein Length : |
Gly48~Gln385 |
Description : |
The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. |
Form : |
Freeze-dried powder |
Molecular Mass : |
66kDa as determined by SDS-PAGE reducing conditions |
Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
Purity : |
>98% |
Characteristic : |
The isoelectric point is 8.6. |
Applications : |
Positive Control; Immunogen; SDS-PAGE; WB |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : |
200μg/mL |
Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. |
Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |