Active Recombinant Human PDCD1 Protein, His-Avi-tagged, Biotinylated
Cat.No. : | PDCD1-259H |
Product Overview : | Biotinylated Human PD-1 is expressed from HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # Q15116-1). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Predicted N Terminal : | Leu 25 |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Nivolumab at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, His Tag with a linear range of 0.8-13 ug/mL. |
Molecular Mass : | The protein has a calculated MW of 18.6 kDa. The protein migrates as 35-45 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | PDCD1 programmed cell death 1 [ Homo sapiens ] |
Official Symbol : | PDCD1 |
Synonyms : | PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l; |
Gene ID : | 5133 |
mRNA Refseq : | NM_005018 |
Protein Refseq : | NP_005009 |
MIM : | 600244 |
UniProt ID : | Q15116 |
Products Types
◆ Recombinant Protein | ||
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Pdcd1-4729M | Recombinant Mouse Pdcd1 Protein, DDK/His-tagged | +Inquiry |
PDCD1-1624H | Recombinant Human PDCD1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PDCD1-06H | Active Recombinant Human PDCD1 Protein, hIgG-tagged | +Inquiry |
PDCD1-1033R | Active Recombinant Rat PDCD1 protein(Met1-Gln167), hFc-tagged | +Inquiry |
◆ Lysates | ||
PDCD1-001RCL | Recombinant Rat PDCD1 cell lysate | +Inquiry |
PDCD1-2873HCL | Recombinant Human PDCD1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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